Analysis of distant communication on defined chromatin templates in vitro

Yury S. Polikanov, Vasily M. Studitsky

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

8 Scopus citations

Abstract

Regulation of many biological processes in eukaryotes involves distant communication between the regulatory DNA sequences (e.g., enhancers) and their targets over the DNA regions organized in chromatin. However previously developed methods for analysis of communication in chromatin in vitro are artifact-prone and/or do not allow analysis of communication on physiologically relevant, saturated arrays of nucleosomes. Here we describe a method for quantitative analysis of the rate of distant communication in cis on saturated arrays of nucleosomes capable of forming the 30-nm chromatin fibers in vitro.

Original languageEnglish
Title of host publicationDNA-Protein Interactions
Subtitle of host publicationPrinciples and Protocols, Third Edition
EditorsTom Moss, Benoit Leblanc
Pages563-576
Number of pages14
DOIs
StatePublished - 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume543
ISSN (Print)1064-3745

Keywords

  • Chromatin
  • Enhancer
  • Nucleosome
  • Promoter
  • Transcription

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