TY - JOUR
T1 - Analysis of cyclin E and CDK2 in ovarian cancer
T2 - Gene amplification and RNA overexpression
AU - Marone, Maria
AU - Scambia, Giovanni
AU - Giannttelli, Cecilia
AU - Ferrandina, Gabriella
AU - Masciullo, Valeria
AU - Bellacosa, Alfonso
AU - Benedetti-Panici, Pierluigi
AU - Mancuso, Salvatore
PY - 1998/1/5
Y1 - 1998/1/5
N2 - Cyclins and their associated kinases (cdks) play a key role in controlling the cell cycle, a process whose disregulation can potentially lead to uncontrolled cell growth and hence to cancer. We have studied the role of both cyclin E and its associated kinase cdk2 in ovarian cancer. Primary, metastatic, recurrent and benign ovarian tumors were screened for cyclin E and cdk2 gene amplification. Cyclin E was shown to be amplified in 21% and cdk2 in 6.4% of the cases analyzed. Cyclin E and cdk2 RNA expression levels were determined by semi-quantitative RT-PCR analysis in a partially overlapping series of samples and compared to the expression levels of normal ovarian surface epithelial cells. Cyclin E RNA was overexpressed in 29.5% and cdk2 in 6.5% of ovarian tumors tested. We determined that in most cases gene amplification leads to higher RNA levels for cyclin E and that the overall levels of cyclin E and cdk2 RNA were correlated. We hypothesize that cyclin E and cdk2 are, in part co-regulated and that they may concur to ovarian tumor development.
AB - Cyclins and their associated kinases (cdks) play a key role in controlling the cell cycle, a process whose disregulation can potentially lead to uncontrolled cell growth and hence to cancer. We have studied the role of both cyclin E and its associated kinase cdk2 in ovarian cancer. Primary, metastatic, recurrent and benign ovarian tumors were screened for cyclin E and cdk2 gene amplification. Cyclin E was shown to be amplified in 21% and cdk2 in 6.4% of the cases analyzed. Cyclin E and cdk2 RNA expression levels were determined by semi-quantitative RT-PCR analysis in a partially overlapping series of samples and compared to the expression levels of normal ovarian surface epithelial cells. Cyclin E RNA was overexpressed in 29.5% and cdk2 in 6.5% of ovarian tumors tested. We determined that in most cases gene amplification leads to higher RNA levels for cyclin E and that the overall levels of cyclin E and cdk2 RNA were correlated. We hypothesize that cyclin E and cdk2 are, in part co-regulated and that they may concur to ovarian tumor development.
KW - Adult
KW - Aged
KW - CDC2-CDC28 Kinases
KW - Cyclin E/genetics
KW - Cyclin-Dependent Kinase 2
KW - Cyclin-Dependent Kinases/genetics
KW - Female
KW - Gene Amplification
KW - Humans
KW - Middle Aged
KW - Ovarian Neoplasms/genetics
KW - Protein Serine-Threonine Kinases/genetics
KW - RNA/analysis
UR - http://www.scopus.com/inward/record.url?scp=0032484533&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-0215(19980105)75:1<34::AID-IJC6>3.0.CO;2-2
DO - 10.1002/(SICI)1097-0215(19980105)75:1<34::AID-IJC6>3.0.CO;2-2
M3 - Article
C2 - 9426687
AN - SCOPUS:0032484533
SN - 0020-7136
VL - 75
SP - 34
EP - 39
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 1
ER -
