An Epitope-Specific Respiratory Syncytial Virus Vaccine Based on an Antibody Scaffold

Xiaozhou Luo; Tao Liu; Ying Wang; Haiqun Jia; Yuhan Zhang; Dawna Caballero; Juanjuan Du; Rongsheng E. Wang; Danling Wang; Peter G. Schultz; Feng Wang

doi:10.1002/anie.201507928

An Epitope-Specific Respiratory Syncytial Virus Vaccine Based on an Antibody Scaffold

Xiaozhou Luo
, Tao Liu
, Ying Wang
, Haiqun Jia
, Yuhan Zhang
, Dawna Caballero
, Juanjuan Du
, Rongsheng E. Wang
, Danling Wang
, Peter G. Schultz
, Feng Wang

Scripps Research Institute
California Institute for Biomedical Research

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract infections in children. We have generated an epitope-specific RSV vaccine by grafting a neutralizing epitope (F-epitope) in its native conformation into an immunoglobulin scaffold. The resulting antibody fusion exhibited strong binding affinity to Motavizumab, an RSV neutralizing antibody, and effectively induced potent neutralizing antibodies in mice. This work illustrates the potential of the immunoglobulin molecule as a scaffold to present conformationally constrained B-cell epitopes.

Original language	English
Pages (from-to)	14531-14534
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	54
Issue number	48
DOIs	https://doi.org/10.1002/anie.201507928
State	Published - Nov 23 2015
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

RSV vaccine
antibodies
conformations
polypeptides
protein engineering

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10.1002/anie.201507928

Cite this

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title = "An Epitope-Specific Respiratory Syncytial Virus Vaccine Based on an Antibody Scaffold",

abstract = "Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract infections in children. We have generated an epitope-specific RSV vaccine by grafting a neutralizing epitope (F-epitope) in its native conformation into an immunoglobulin scaffold. The resulting antibody fusion exhibited strong binding affinity to Motavizumab, an RSV neutralizing antibody, and effectively induced potent neutralizing antibodies in mice. This work illustrates the potential of the immunoglobulin molecule as a scaffold to present conformationally constrained B-cell epitopes.",

keywords = "RSV vaccine, antibodies, conformations, polypeptides, protein engineering",

author = "Xiaozhou Luo and Tao Liu and Ying Wang and Haiqun Jia and Yuhan Zhang and Dawna Caballero and Juanjuan Du and Wang, \{Rongsheng E.\} and Danling Wang and Schultz, \{Peter G.\} and Feng Wang",

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doi = "10.1002/anie.201507928",

language = "English",

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journal = "Angewandte Chemie - International Edition",

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T1 - An Epitope-Specific Respiratory Syncytial Virus Vaccine Based on an Antibody Scaffold

AU - Luo, Xiaozhou

AU - Liu, Tao

AU - Wang, Ying

AU - Jia, Haiqun

AU - Zhang, Yuhan

AU - Caballero, Dawna

AU - Du, Juanjuan

AU - Wang, Rongsheng E.

AU - Wang, Danling

AU - Schultz, Peter G.

AU - Wang, Feng

PY - 2015/11/23

Y1 - 2015/11/23

N2 - Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract infections in children. We have generated an epitope-specific RSV vaccine by grafting a neutralizing epitope (F-epitope) in its native conformation into an immunoglobulin scaffold. The resulting antibody fusion exhibited strong binding affinity to Motavizumab, an RSV neutralizing antibody, and effectively induced potent neutralizing antibodies in mice. This work illustrates the potential of the immunoglobulin molecule as a scaffold to present conformationally constrained B-cell epitopes.

AB - Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract infections in children. We have generated an epitope-specific RSV vaccine by grafting a neutralizing epitope (F-epitope) in its native conformation into an immunoglobulin scaffold. The resulting antibody fusion exhibited strong binding affinity to Motavizumab, an RSV neutralizing antibody, and effectively induced potent neutralizing antibodies in mice. This work illustrates the potential of the immunoglobulin molecule as a scaffold to present conformationally constrained B-cell epitopes.

KW - RSV vaccine

KW - antibodies

KW - conformations

KW - polypeptides

KW - protein engineering

UR - https://www.scopus.com/pages/publications/84954357450

U2 - 10.1002/anie.201507928

DO - 10.1002/anie.201507928

M3 - Article

C2 - 26434555

SN - 1433-7851

VL - 54

SP - 14531

EP - 14534

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 48

ER -

An Epitope-Specific Respiratory Syncytial Virus Vaccine Based on an Antibody Scaffold

Abstract

UN SDGs

Keywords

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