Abstract
We isolated cDNA clones containing the entire coding region of the putative oncogene AKT2. Sequence analysis and in vitro translation demonstrated that AKT2 encodes a 56-kDa protein with homology to serine/threonine kinases; moreover, this protein contains a Src homology 2-like domain. AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. AKT2 was mapped to chromosome region 19q13.1-q13.2 by fluorescence in situ hybridization. In the two ovarian carcinoma cell lines exhibiting amplification of AKT2, the amplified sequences were localized within homogeneously staining regions. We conclude that AKT2 belongs to a distinct subfamily of protein-serine/threonine kinases containing Src homology 2-like domains and that alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas.
Original language | English |
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Pages (from-to) | 9267-9271 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 89 |
Issue number | 19 |
DOIs | |
State | Published - Oct 1 1992 |
Keywords
- Amino Acid Sequence
- Chromosomes, Human/ultrastructure
- Cloning, Molecular
- DNA/genetics
- Female
- Fluorescent Dyes
- Gene Amplification
- Gene Library
- Humans
- Lymphocytes/cytology
- Molecular Sequence Data
- Multigene Family
- Oncogene Proteins/genetics
- Oncogenes
- Ovarian Neoplasms/enzymology
- Protein Biosynthesis
- Protein Serine-Threonine Kinases/genetics
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-akt
- Sequence Homology, Amino Acid
- Transcription, Genetic