TY - JOUR
T1 - Adenylyl cyclase isoforms are differentially expressed in primary cultures of endothelial cells and whole tissue-homogenates from various rat tissues
AU - Manolopoulos, Vangelis G.
AU - Liu, Jian Dong
AU - Unsworth, Brian R.
AU - Lelkes, Peter I.
PY - 1995/3/8
Y1 - 1995/3/8
N2 - The expression of five adenylyl cyclase isoforms (types II-VI) was studied with reverse transcription-polymerase chain reaction in whole tissue homogenates and in primary cultures of endothelial cells isolated from rat aorta, vena cava, heart, lung, adipose fat, brain, and adrenal medulla. It was found that: i) all endothelial cell types express all adenylyl cyclase isoforms studied, albeit at different levels depending on the tissue origin of the cells, and ii) the adenylyl cyclase isoform profile of isolated endothelial cells differs from that of homogenates of their parent tissues. Our data show a unique endothelial cell type specificity of AC isoform expression, which varies from that of the whole organ. These results support the idea that one of the factors mediating differential regulation of the cAMP cascade in EC in various locations within the vascular tree might be quantitative differences in the levels of AC isoforms expressed in each EC type.
AB - The expression of five adenylyl cyclase isoforms (types II-VI) was studied with reverse transcription-polymerase chain reaction in whole tissue homogenates and in primary cultures of endothelial cells isolated from rat aorta, vena cava, heart, lung, adipose fat, brain, and adrenal medulla. It was found that: i) all endothelial cell types express all adenylyl cyclase isoforms studied, albeit at different levels depending on the tissue origin of the cells, and ii) the adenylyl cyclase isoform profile of isolated endothelial cells differs from that of homogenates of their parent tissues. Our data show a unique endothelial cell type specificity of AC isoform expression, which varies from that of the whole organ. These results support the idea that one of the factors mediating differential regulation of the cAMP cascade in EC in various locations within the vascular tree might be quantitative differences in the levels of AC isoforms expressed in each EC type.
KW - Adenylyl Cyclases/biosynthesis
KW - Adipose Tissue/blood supply
KW - Animals
KW - Aorta
KW - Base Sequence
KW - Cells, Cultured
KW - Coronary Vessels
KW - DNA Primers
KW - Endothelium, Vascular/enzymology
KW - Gene Expression Regulation, Enzymologic
KW - Glyceraldehyde-3-Phosphate Dehydrogenases/biosynthesis
KW - Isoenzymes/biosynthesis
KW - Lung/blood supply
KW - Microcirculation
KW - Molecular Sequence Data
KW - Organ Specificity
KW - Polymerase Chain Reaction
KW - Rats
KW - Rats, Sprague-Dawley
KW - Venae Cavae
UR - http://www.scopus.com/inward/record.url?scp=0028930949&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:A1995QL17200045&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1006/bbrc.1995.1341
DO - 10.1006/bbrc.1995.1341
M3 - Article
C2 - 7887946
SN - 0006-291X
VL - 208
SP - 323
EP - 331
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -