A role for Ly108 in the induction of promyelocytic zinc finger transcription factor in developing thymocytes

Mala Dutta; Zachary J. Kraus; Julio Gomez-Rodriguez; Sun Hee Hwang; Jennifer L. Cannons; Jun Cheng; Sang Yun Lee; David L. Wiest; Edward K. Wakeland; Pamela L. Schwartzberg

doi:10.4049/jimmunol.1202145

A role for Ly108 in the induction of promyelocytic zinc finger transcription factor in developing thymocytes

Mala Dutta, Zachary J. Kraus, Julio Gomez-Rodriguez, Sun Hee Hwang, Jennifer L. Cannons, Jun Cheng, Sang Yun Lee, David L. Wiest, Edward K. Wakeland, Pamela L. Schwartzberg

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

The promyelocytic zinc finger transcription factor (PLZF) is required for the development of activated phenotypes in NKT and other innate T lymphocytes. Although strong TCR stimulation has been implicated in the induction of PLZF, the factors regulating PLZF expression are incompletely understood.We show in this study that costimulation of preselection double-positive thymocytes through the signaling lymphocyte activation molecule family receptor Ly108 markedly enhanced PLZF expression compared with that induced by TCR stimulation alone. Costimulation with Ly108 increased expression of early growth response protein (Egr)-2 and binding of Egr-2 to the promoter of Zbtb16, which encodes PLZF, and resulted in PLZF levels similar to those seen in NKT cells. In contrast, costimulation with anti-CD28 failed to enhance Egr-2 binding and Zbtb16 expression. Moreover, mice lacking Ly108 showed decreased numbers of PLZF-expressing CD4⁺ T cells. Together, these results support a potential role for Ly108 in the induction of PLZF.

Original language	English
Pages (from-to)	2121-2128
Number of pages	8
Journal	Journal of Immunology
Volume	190
Issue number	5
DOIs	https://doi.org/10.4049/jimmunol.1202145
State	Published - Mar 1 2013

Keywords

Animals
Antibodies/pharmacology
Antigens, Ly/genetics
CD28 Antigens/antagonists & inhibitors
CD4 Lymphocyte Count
CD4-Positive T-Lymphocytes/cytology
Calcium/metabolism
Cell Differentiation/drug effects
Cells, Cultured
Early Growth Response Protein 2/genetics
Gene Expression Regulation/drug effects
Kruppel-Like Transcription Factors/genetics
Lipopolysaccharides/pharmacology
Lymphocyte Activation/drug effects
Mice
Mice, Transgenic
Promoter Regions, Genetic
Promyelocytic Leukemia Zinc Finger Protein
Receptors, Antigen, T-Cell/genetics
Signal Transduction/drug effects
Thymocytes/cytology

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title = "A role for Ly108 in the induction of promyelocytic zinc finger transcription factor in developing thymocytes",

abstract = "The promyelocytic zinc finger transcription factor (PLZF) is required for the development of activated phenotypes in NKT and other innate T lymphocytes. Although strong TCR stimulation has been implicated in the induction of PLZF, the factors regulating PLZF expression are incompletely understood.We show in this study that costimulation of preselection double-positive thymocytes through the signaling lymphocyte activation molecule family receptor Ly108 markedly enhanced PLZF expression compared with that induced by TCR stimulation alone. Costimulation with Ly108 increased expression of early growth response protein (Egr)-2 and binding of Egr-2 to the promoter of Zbtb16, which encodes PLZF, and resulted in PLZF levels similar to those seen in NKT cells. In contrast, costimulation with anti-CD28 failed to enhance Egr-2 binding and Zbtb16 expression. Moreover, mice lacking Ly108 showed decreased numbers of PLZF-expressing CD4+ T cells. Together, these results support a potential role for Ly108 in the induction of PLZF.",

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author = "Mala Dutta and Kraus, {Zachary J.} and Julio Gomez-Rodriguez and Hwang, {Sun Hee} and Cannons, {Jennifer L.} and Jun Cheng and Lee, {Sang Yun} and Wiest, {David L.} and Wakeland, {Edward K.} and Schwartzberg, {Pamela L.}",

year = "2013",

month = mar,

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doi = "10.4049/jimmunol.1202145",

language = "English",

volume = "190",

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journal = "Journal of Immunology",

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T1 - A role for Ly108 in the induction of promyelocytic zinc finger transcription factor in developing thymocytes

AU - Dutta, Mala

AU - Kraus, Zachary J.

AU - Gomez-Rodriguez, Julio

AU - Hwang, Sun Hee

AU - Cannons, Jennifer L.

AU - Cheng, Jun

AU - Lee, Sang Yun

AU - Wiest, David L.

AU - Wakeland, Edward K.

AU - Schwartzberg, Pamela L.

PY - 2013/3/1

Y1 - 2013/3/1

N2 - The promyelocytic zinc finger transcription factor (PLZF) is required for the development of activated phenotypes in NKT and other innate T lymphocytes. Although strong TCR stimulation has been implicated in the induction of PLZF, the factors regulating PLZF expression are incompletely understood.We show in this study that costimulation of preselection double-positive thymocytes through the signaling lymphocyte activation molecule family receptor Ly108 markedly enhanced PLZF expression compared with that induced by TCR stimulation alone. Costimulation with Ly108 increased expression of early growth response protein (Egr)-2 and binding of Egr-2 to the promoter of Zbtb16, which encodes PLZF, and resulted in PLZF levels similar to those seen in NKT cells. In contrast, costimulation with anti-CD28 failed to enhance Egr-2 binding and Zbtb16 expression. Moreover, mice lacking Ly108 showed decreased numbers of PLZF-expressing CD4+ T cells. Together, these results support a potential role for Ly108 in the induction of PLZF.

AB - The promyelocytic zinc finger transcription factor (PLZF) is required for the development of activated phenotypes in NKT and other innate T lymphocytes. Although strong TCR stimulation has been implicated in the induction of PLZF, the factors regulating PLZF expression are incompletely understood.We show in this study that costimulation of preselection double-positive thymocytes through the signaling lymphocyte activation molecule family receptor Ly108 markedly enhanced PLZF expression compared with that induced by TCR stimulation alone. Costimulation with Ly108 increased expression of early growth response protein (Egr)-2 and binding of Egr-2 to the promoter of Zbtb16, which encodes PLZF, and resulted in PLZF levels similar to those seen in NKT cells. In contrast, costimulation with anti-CD28 failed to enhance Egr-2 binding and Zbtb16 expression. Moreover, mice lacking Ly108 showed decreased numbers of PLZF-expressing CD4+ T cells. Together, these results support a potential role for Ly108 in the induction of PLZF.

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KW - Cell Differentiation/drug effects

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KW - Gene Expression Regulation/drug effects

KW - Kruppel-Like Transcription Factors/genetics

KW - Lipopolysaccharides/pharmacology

KW - Lymphocyte Activation/drug effects

KW - Mice

KW - Mice, Transgenic

KW - Promoter Regions, Genetic

KW - Promyelocytic Leukemia Zinc Finger Protein

KW - Receptors, Antigen, T-Cell/genetics

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DO - 10.4049/jimmunol.1202145

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C2 - 23355739

SN - 0022-1767

VL - 190

SP - 2121

EP - 2128

JO - Journal of Immunology

JF - Journal of Immunology

IS - 5

ER -

A role for Ly108 in the induction of promyelocytic zinc finger transcription factor in developing thymocytes

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