TY - JOUR
T1 - A new human synovial sarcoma cell line, HS-SY-3, with a truncated form of hybrid SYT/SSX1 gene
AU - Sonobe, Hiroshi
AU - Takeuchi, Tamotsu
AU - Liag, Sheng Ben
AU - Taguchi, Takahiro
AU - Yuri, Kazunari
AU - Shimizu, Kiichi
AU - Iwata, Jun
AU - Furihata, Mutsuo
AU - Ohtsuki, Yuji
AU - Testa, Joseph R.
PY - 1999
Y1 - 1999
N2 - Recent cytogenetical and molecular studies have indicated that synovial sarcoma harbors a t(X;18)(p11.2;q11.2) translocation, resulting in the formation of a hybrid SYT/SSX (SSX1 or SSX2) gene. We newly established a human cell line, HS-SY-3, from a synovial sarcoma. HS-SY-3 cells were shown to harbor the pathognomonic t(X;18)(p11.2;q11.2) translocation by chromosome analysis but not to exhibit the classical hybrid SYT/SSX transcripts induced by this translocation, using RTPCR. To determine the reason for this discrepancy, we analyzed cDNA from HS-SY-3 cells, as well as the original sarcoma tissue by the rapid amplification of cDNA 3' end assay, and found that the chimaeric cDNA was 240 bp shorter than the previously established SYT/SSX1 cDNA due to truncation of the 3' side of SSX1. The HS-SY-3 cells should be useful for future functional studies of the SYT/SSX chimeric gene.
AB - Recent cytogenetical and molecular studies have indicated that synovial sarcoma harbors a t(X;18)(p11.2;q11.2) translocation, resulting in the formation of a hybrid SYT/SSX (SSX1 or SSX2) gene. We newly established a human cell line, HS-SY-3, from a synovial sarcoma. HS-SY-3 cells were shown to harbor the pathognomonic t(X;18)(p11.2;q11.2) translocation by chromosome analysis but not to exhibit the classical hybrid SYT/SSX transcripts induced by this translocation, using RTPCR. To determine the reason for this discrepancy, we analyzed cDNA from HS-SY-3 cells, as well as the original sarcoma tissue by the rapid amplification of cDNA 3' end assay, and found that the chimaeric cDNA was 240 bp shorter than the previously established SYT/SSX1 cDNA due to truncation of the 3' side of SSX1. The HS-SY-3 cells should be useful for future functional studies of the SYT/SSX chimeric gene.
KW - Aged
KW - Aged, 80 and over
KW - Female
KW - Gene Amplification
KW - Humans
KW - Hybridization, Genetic
KW - In Situ Hybridization, Fluorescence
KW - Karyotyping
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Sarcoma, Synovial/genetics
KW - Translocation, Genetic
KW - Tumor Cells, Cultured
UR - http://www.scopus.com/inward/record.url?scp=0032970157&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:000081189200021&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1002/(SICI)1097-0215(19990730)82:3<459::AID-IJC21>3.0.CO;2-B
DO - 10.1002/(SICI)1097-0215(19990730)82:3<459::AID-IJC21>3.0.CO;2-B
M3 - Article
C2 - 10399965
SN - 0020-7136
VL - 82
SP - 459
EP - 464
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 3
ER -