TY - JOUR
T1 - A homogeneous fluorescent sensor for human serum albumin
AU - Ross, Rongsheng E.
AU - Tian, Ling
AU - Chang, Yie Hwa
N1 - Copyright © 2012 Elsevier B.V. All rights reserved.
PY - 2012/4/7
Y1 - 2012/4/7
N2 - Human serum albumin is the most abundant protein in the body and is an important biomarker used for disease-related diagnosis. Although the traditional enzyme-linked immunosorbent assay (ELISA) approach can precisely measure the concentration of human serum albumin, the multi-step procedure and time-consuming preparations of ELISA limit its diagnostic applications, preventing accurate point-of-care testing, for example. Herein, we report the recent development of an antibody-based albumin sensor that allows for a homogeneous measurement of albumin concentrations in saliva, urine and serum, in which this type of sensor is validated for the first time. The assay only requires simple mixing, and relies on time-resolved (TR) fluorescence resonance energy transfer (FRET) to produce robust, sensitive signals. The whole process, from sample preparation to final read-out, is expected to take less than 1. h and requires only a standard plate-reader, thus making the sensor a convenient and cost-effective tool for albumin analysis.
AB - Human serum albumin is the most abundant protein in the body and is an important biomarker used for disease-related diagnosis. Although the traditional enzyme-linked immunosorbent assay (ELISA) approach can precisely measure the concentration of human serum albumin, the multi-step procedure and time-consuming preparations of ELISA limit its diagnostic applications, preventing accurate point-of-care testing, for example. Herein, we report the recent development of an antibody-based albumin sensor that allows for a homogeneous measurement of albumin concentrations in saliva, urine and serum, in which this type of sensor is validated for the first time. The assay only requires simple mixing, and relies on time-resolved (TR) fluorescence resonance energy transfer (FRET) to produce robust, sensitive signals. The whole process, from sample preparation to final read-out, is expected to take less than 1. h and requires only a standard plate-reader, thus making the sensor a convenient and cost-effective tool for albumin analysis.
KW - Enzyme-linked immunosorbent assay (ELISA)
KW - Human serum albumin (HSA)
KW - Sensor
KW - Time-resolved fluorescence energy transfer (TR-FRET)
UR - http://www.scopus.com/inward/record.url?scp=84862822832&partnerID=8YFLogxK
U2 - 10.1016/j.jpba.2011.12.035
DO - 10.1016/j.jpba.2011.12.035
M3 - Article
C2 - 22326845
SN - 0731-7085
VL - 63
SP - 165
EP - 169
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
ER -