As a graduate student at MIT, Dr. Golemis identified enhancer elements driving leukemia virus pathogenicity. As a postdoctoral fellow at Massachusetts General Hospital and Harvard Medical School, she led the development of a two-hybrid system to identify and define protein interactions. Now, as the William Wikoff Smith Chair in Cancer Biology at Fox Chase Cancer Center in Philadelphia, she serves as Associate Director for System Integration; she also holds a second role as Chair of the Department of Cancer and Cellular Biology at the Lewis Katz School of Medicine at Temple University.

At Fox Chase, her research focuses on the study of complex cancer signaling networks that influence tumor progression and therapeutic response, and employs approaches from bioinformatics, high throughput screening, and classic cell and molecular biological approaches. Dr. Golemis is a Fellow of the American Association for the Advancement of Science. She has published over 240 articles and edited 4 books on topics in cancer and biotechnology.