Research output per year
Research output per year
Research Resources Details
Description
The Transgenic Mouse Facility (TMF) provides Cancer Center members the ability to introduce manipulated genes into the murine germline, and to delete or modify endogenous murine genes. Production of transgenic mice is an essential technology that enables investigators to answer questions about gene function that can only be addressed in a whole animal model system.
Primary Objectives:
1. Generation of transgenic mice by pronuclear injection of DNA constructs into mouse zygotes.
2. Generation of knockout mice by injection of CRISPR reagents into mouse zygotes.
3. Embryo cryopreservation and revival of mouse strains from frozen embryos.
This facility derives partial funding from the FCCC Cancer Center Support Grant (CCSG) from the National Cancer Institute.
Primary Objectives:
1. Generation of transgenic mice by pronuclear injection of DNA constructs into mouse zygotes.
2. Generation of knockout mice by injection of CRISPR reagents into mouse zygotes.
3. Embryo cryopreservation and revival of mouse strains from frozen embryos.
This facility derives partial funding from the FCCC Cancer Center Support Grant (CCSG) from the National Cancer Institute.
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Research output
- 24 Article
-
Genetic separation of Brca1 functions reveal mutation-dependent Polθ vulnerabilities
Krais, J. J., Glass, D. J., Chudoba, I., Wang, Y., Feng, W., Simpson, D., Patel, P., Liu, Z., Neumann-Domer, R., Betsch, R. G., Bernhardy, A. J., Bradbury, A. M., Conger, J., Yueh, W. T., Nacson, J., Pomerantz, R. T., Gupta, G. P., Testa, J. R. & Johnson, N., Dec 2023, In: Nature Communications. 14, 1, p. 7714 7714.Research output: Contribution to journal › Article › peer-review
Open Access7 Scopus citations -
TET1 and TDG Suppress Inflammatory Response in Intestinal Tumorigenesis: Implications for Colorectal Tumors With the CpG Island Methylator Phenotype
Tricarico, R., Madzo, J., Scher, G., Cohen, M., Jelinek, J., Maegawa, S., Nagarathinam, R., Scher, C., Chang, W. C., Nicolas, E., Slifker, M., Zhou, Y., Devarajan, K., Cai, K. Q., Kwok, T., Nakajima, P., Xu, J., Mancuso, P., Doneddu, V. & Bagella, L. & 21 others, , May 2023, In: Gastroenterology. 164, 6, p. 921-936.e1Research output: Contribution to journal › Article › peer-review
Open Access8 Scopus citations -
ThPOK is a critical multifaceted regulator of myeloid lineage development
Basu, J., Olsson, A., Ferchen, K., Titerina, E. K., Chetal, K., Nicolas, E., Czyzewicz, P., Levchenko, D., Ge, L., Hua, X., Grimes, H. L., Salomonis, N. & Kappes, D. J., Aug 2023, In: Nature Immunology. 24, 8, p. 1295-1307 13 p.Research output: Contribution to journal › Article › peer-review
1 Scopus citations